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. 2021 Nov 26;10(12):1895. doi: 10.3390/antiox10121895

Figure 2.

Figure 2

Effects of Papaver nudicaule orange (NO-Fr), Papaver nudicaule scarlet (NS-Fr) and Papaver rhoeas red (RA-Fr) on the expression of PGE2, iNOS and COX2. RAW264.7 cells were treated with NO-Fr, NS-Fr and RA-Fr (12.5 and 25 μg/mL), and lipopolysaccharides (LPS) (5 ng/mL) for 24 h. (a) The PGE2 secretion was analyzed, and data are shown as mean ± standard error of the mean (SEM). (b) The expression of iNOS, COX2 and β-actin in the treated cells was investigated by western blotting. (c,d) Quantification of iNOS, COX2 and β-actin expression was performed using the ImageJ software. Expression levels of iNOS and COX2 were normalized to the β-actin protein levels. Data are shown as mean ± standard deviation (SD). * p < 0.05, ** p < 0.01 and *** p < 0.001 imply statistically significant differences compared with the LPS only treated group.