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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Nat Protoc. 2021 Aug 11;16(10):4676–4691. doi: 10.1038/s41596-021-00592-4

Recipe 1:

lysis buffer

Components Volume
RLT buffer (Qiagen) 965 μL
β-Mercaptoethanol 10 μL
Superase-In, 20 U/uL 15 μL
Chloramphenicol, 50 mg/mL 10 μL
Total 1 mL (scale as needed)