| Reagent | Volume |
|---|---|
| Lysate (from Step 13) | X μL (to 80 μg RNA total) |
| CaCl2, 500 mM | 2 μL |
| Superase-In, 20 U/μL | 2 μL |
| NEB MNase, 500 U/μL | 1 μL |
| MNase buffer | To 200 μL |
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