| Reagent | Volume |
|---|---|
| RNA footprints (from Step 28) | 18 μL |
| 10X T4 PNK buffer | 2.2 μL |
| Superase-In, 20 U/μL | 1 μL |
| T4 PNK, 10 U/μL | 1 μL |
| Total volume | 22.2 μL |
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