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. 2021 Dec 22;11(1):22. doi: 10.3390/cells11010022

Figure 5.

Figure 5

HuR expression in non-smoker, smoker, and COPD HLFs. (A) ELAVL1 mRNA-RT-qPCR: HLFs from 4 non-smoker (circle), 4 smoker (square), and 4 COPD (triangle) subjects were harvested for RT-qPCR. There was a significant difference between smoker and COPD-derived HLFs (** p < 0.01). Results are expressed as the mean ± SEM. (B) Total HuR Protein—Western blot- densitometry: HLFs from 4 non-smoker, 4 smoker, and 4 COPD subjects were harvested for Western blot. HuR protein expression was detected at the predicted MW of 34 kDa. The cleaved HuR product (CP-1, 27 kDa) was detected in HLFs from smoker and COPD subjects. β-Tubulin was used as loading control. (C) Total HuR Protein—densitometry: there was no significant difference in total expression of HuR between non-smoker (circle), smoker (square), and COPD (triangle) HLFs. Results are expressed as the mean ± SEM. (D) Cleaved HuR Protein (CP-1)—densitometry: there was significant increase in the cleaved HuR (CP-1) in smoker (square) HLFs comparing to non-smoker (circle) HLFs (** p = 0.009). Results are expressed as the mean ± SEM. (E). Cleaved HuR in non-smoker HLFs exposed to 2% CSE: there was an increase in the cleaved HuR product (CP-1) in HLFs from non-smokers exposed to 2% CSE for 8 h and 24 h (* p = 0.01). Results are expressed as the mean ± SEM of 4 independent experiments.