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. 2021 Dec 22;11(1):22. doi: 10.3390/cells11010022

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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ACE2 mRNA expression and binding to HuR. (A) ACE2-201-202 mRNA: there was significantly less mRNA in smoker (triangle) compared to non-smoker (circle) HLFs (* p < 0.05) (B) ACE2-202 mRNA: there was no significant difference between the three groups of HLFs. (C) RIP—Western blot: representative Western blot of HuR IP is shown. Input (In) refers to cell lysates. IP-IgG refers to immunoprecipitation (IP) with control IgG antibody while IP-HuR refers to the IP with anti-HuR IgG antibody. Note the presence of HuR protein in IP-HuR but not in IP-IgG. (D) ACE2-201-202 mRNA: detection of ACE2-201-202 mRNA in IP-IgG and IP-HuR was done using qPCR. Values are expressed as fold enrichment to values measured in IP-IgG. The enrichment of ACE2-201-202 in IP-HuR of HLFs from non-smoker and smoker but not in COPD. Results are presented as the mean ± SEM) (* p < 0.05 compared to IP-IgG).