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. 2021 Dec 22;11(1):22. doi: 10.3390/cells11010022

Figure 9.

Figure 9

HuR silencing does not affect protein and mRNA expression of ACE2. (A) HuR protein—Western blot: transfection of non-smoker HLFs with esiHuR reduced the level of HuR protein by ~80%. (B) ACE2 protein: there was no significant difference in ACE2 protein levels in esiHuR-transfected HLFs exposed to 2% CSE for 24 h compared to untreated esiHuR-transfected cells and to esiCtrl-transfected cells. Results are expressed as the mean ± SEM of 3 independent experiments (HLFs used from one non-smoker subject). (C) ACE2 mRNA: there was no significant difference in ACE2 mRNA in esiHuR-transfected HLFs exposed to 2% CSE for 24 h compared to untreated esiHuR-transfected cells and to esiCtrl-transfected cells. Results are expressed as the mean ± SEM of 3 independent experiments (HLFs used from one non-smoker subject).