Table 1:
Comparison of the motif-strengthening mutants and the wildtype systems. For each mutant, the mutations, the 3D prediction programs (R for RNAComposer, S for SimRNA, I for iFoldRNA, V for Vfold3D), the wildtype and mutant Stem 2 lengths, and the newly formed Stem 2 base pairs involving the mutated residues are listed.
| Program | WT S2 | Mutant S2 | Base pairs involving mutations |
|---|---|---|---|
| 77-nt 3_6 PSM [G3U, U4A, G18A, C19A, C68A, A69C] | |||
| R | 4 | 4 | G25-C69 |
| S | 4 | 9 | A18-U76, A19-U75, G25-C69, U26-A68 |
| I | 7 | 7 | A19-U75, G25-C69 |
| V | 4 | 8 | A18-U76, A19-U75, G25-C69, U26-A68 |
| 144-nt 3_6 PSM [G40U, U41A, G55A, C56A, C105A, A106C, C137A] | |||
| R | 4 | 5 | G62-C106 |
| I | 5 | 4 | A56-U112 |
| 77-nt 3_3 PSM [U4C, G71A, G72U] | |||
| I | 3 | 7 | C4-G21 |
| 77-nt 3_5 Mutant [G72C, U74C] | |||
| R | 3 | 7 | G1-C74, G3-C72 |
| S | 3 | 7 | G1-C74, G3-C72 |
| I | 4 | 7 | G1-C74, G3-C72 |
| V | 3 | 6 | G3-C72 |