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. 2021 Dec 21;14(1):16. doi: 10.3390/cancers14010016

Figure 1.

Figure 1

Non protective NLC for CLL cells harbour an M1-like phenotype. (A,B). Flow cytometry analysis of the percentage of viability (Annexin V/7-AAD negative cells) of CLL cells from cultures of PBMC from CLL patients after 14 days. (One representative experiment (A) and data for PBMC from 35 CLL patients separated in two groups, one with high CLL cells viability and one with low CLL cells viability (B)). (C–E) Surface markers expressed by NLC analysed by flow cytometry at 14 days of cultures of PBMC from CLL patients with low (red) or high (green) in vitro CLL cells viability, comparing to the unstained (US: white) and the isotypic (IC: blue) controls (D) one representative experiment; (E) compilation of MFI ratios (marker/isotypic control) of five donors. (F) Fluorescence imaging of co-cultures of NLC (red staining) and CLL cells (green staining), at 1 min and 120 min, for patients with high (left) and low (right) in vitro viability of CLL cells. * indicates p < 0.05.