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AMPK/SIRT1 activation improved autophagy in D-Gal-induced aging mouse heart tissue. (A–G) Representative images from the immunoblot assay against LC3-II/LC3-I ratio, BECN1, SQSTM1, and GAPDH from heart tissue. Expression levels of proteins were quantified using the ImageJ software. All data are presented as mean ± SD (n = 3). * p < 0.05; ** p < 0.01.
