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. 2021 Dec 22;11(1):12. doi: 10.3390/cells11010012

Figure 1.

Figure 1

miR-140-5p and Nrf2 expression in BC cells under hypoxia. (A) Venn diagram showing common miRNAs computationally predicted to target Nrf2 mRNA by all three algorithms (Target Scan, miRDB, and miRSystem). (B) Schematic of the predicted binding site of all four miRNAs in the 3′-UTR of Nrf2 mRNA. (C) qRT-PCR analysis of all four selected miRNAs in MCF-7 and MDA-MB-231 under hypoxic conditions. (D) Relative miR-140-5p expression in normal (n = 76) and primary BC (n = 749) samples based on the data from The Cancer Genome Atlas (TCGA) using the ULCAN web. The survival rates of patients with BC having low (Black) or high (red) miR-140-5p expression as estimated by the Kaplan-Meier method using the data from TCGA. (E,F) Nrf2 and its downstream proteins were analyzed by Western blotting and densitometry analysis using Image J software in MCF-7 and MDA-MB-231. Error bars indicate mean ± SEM (n = 3). Student’s t-tests were used to compare the means of two groups. ns: not significant, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to Normoxia).