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. 2021 Dec 22;11(1):12. doi: 10.3390/cells11010012

Figure 6.

Figure 6

miR-140-5p inhibits BC cell migration and invasion. (A,B) Scratch wound healing assay was carried out to determine the migration ability of MDA-MB-231 cells with miR-140-5p-KD under normoxia (A) or miR-140-5p-OE under hypoxia (B) along with their EV control. (C–E) Transwell migration and invasion assay were performed to determine the migration and invasion ability of MDA-MB-231cells with miR-140-5p-KD under normoxia (C), miR-140-5p-OE under hypoxia (D), or Nrf2-KD under hypoxia (E) along with their controls. (F) Gelatin zymography to analyze MMP-9 and MMP-2 activity using CM from miR-140-5p-KD cells under normoxia or miR-140-5p-OE cells from hypoxia. (G) Analysis of vimentin expression by Western blotting in miR-140-5p-KD cells under normoxia and miR-140-5p-OE cells or Nrf2-KD cells under hypoxia. Error bars indicate mean ± SEM (n = 3). Student’s t-tests were used to compare the means of two groups. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared to EV.