Figure 9.

Estimated values of V₀, V_max, apparent K_Ca, peak Ca²⁺ stimulated state 3 JO₂, and [Ca²⁺]_free required for achieving the peak Ca²⁺ stimulated state 3 JO₂, and their corresponding 95% confidence intervals obtained based on the nonlinear regression (fitting) of Equation (1) and the state 3 JO₂ data from Figure 5. Bar plots in the upper panels (A–E) and bottom panels (F–J) represent the parameters for the heart and kidney OM mitochondria, respectively, for different respiratory substrates (PM, GM, AM, PCM, and SUC + ROT). The peak Ca²⁺ stimulated state 3 JO₂ (D,I) and [Ca²⁺]_free required for achieving the Ca²⁺ peak stimulated state 3 JO₂ (E,J) for each substrate and tissue was predicted by the fitting.