Table 1.

List of plasmid backbones and Synechocystis strains used in this study

Construct	Comment	Antibiotics resistance	References
pEEK	RSF 1010-based expression vector with ccdB flanked by cloning sites and the ribosome binding site RBS*, promoter Ptrc2O, and a C-terminal Strep-tag sequence	Km	[37]
pEEK*	Same as pEEK but without ccdB, used as empty vector control	Km	Englund, E., not published
pEEKN	Same as pEEK but with N-terminal Strep-tag sequence	Km	This study
pEEK3	Based on pEEC3 [38], with PtrcCore promoter, RiboJ and N-terminal Strep-tag sequence, but with Km resistance	Km	Englund, E., not published
pEEK3C	Same as pEEK3 but with C-terminal Strep-tag sequence	Km	This study
pEERM3	Empty backbone based on pJ344 vector backbone for integration into neutral site 1 (slr1068) of Synechocystis 6803, carrying PnrsB promotor and RBS*	Cm	[39]
p1573	Same as pEERM3 but with homologous recombination regions to slr1573	Cm	This study

Strains	Comment	Antibiotics resistance	References
EvC	Control strain of Synechocystis WT harboring pEEK* plasmid for Km resistance	Km	This study
PcC	Petroselinum crispum PAL expressed from Ptrc RBS* in pEEK	Km	This study
AtC	Arabidopsis thaliana PAL expressed from Ptrc RBS* in pEEK	Km	This study
NpC	Nostoc punctiforme PAL expressed from Ptrc RBS* in pEEK	Km	This study
AvC	Anabaena variabilis PAL expressed from Ptrc RBS* in pEEK	Km	This study
TsC	Treponema socranskii PAL expressed from Ptrc RBS* in pEEK	Km	This study
TsC-H87L	Treponema socranskii PAL with mutation H87L expressed from Ptrc RBS* in pEEK	Km	This study
PcN	Petroselinum crispum PAL expressed from Ptrc RBS* in pEEKN	Km	This study
AtN	Arabidopsis thaliana PAL expressed from Ptrc RBS* in pEEKN	Km	This study
NpN	Nostoc punctiforme PAL expressed from Ptrc RBS* in pEEKN	Km	This study
AvN	Anabaena variabilis PAL expressed from Ptrc RBS* in pEEKN	Km	This study
TsN	Treponema socranskii PAL expressed from Ptrc RBS* in pEEKN	Km	This study
TsN-H87L	Treponema socranskii PAL with mutation H87L expressed from Ptrc RBS* in pEEKN	Km	This study
Pc3C	Petroselinum crispum PAL expressed from Ptrc RiboJ in pEEK3C	Km	This study
At3C	Arabidopsis thaliana PAL expressed from Ptrc RiboJ in pEEK3C	Km	This study
Np3C	Nostoc punctiforme PAL expressed from Ptrc RiboJ in pEEK3C	Km	This study
Av3C	Anabaena variabilis PAL expressed from Ptrc RiboJ in pEEK3C	Km	This study
Ts3C	Treponema socranskii PAL expressed from Ptrc RiboJ in pEEK3C	Km	This study
Ts3C-H87L	Treponema socranskii PAL with mutation H87L expressed from Ptrc RiboJ in pEEK3C	Km	This study
Pc3N	Petroselinum crispum PAL expressed from Ptrc RiboJ in pEEK3	Km	This study
At3N	Arabidopsis thaliana PAL expressed from Ptrc RiboJ in pEEK3	Km	This study
Np3N	Nostoc punctiforme PAL expressed from Ptrc RiboJ in pEEK3	Km	This study
Av3N	Anabaena variabilis PAL expressed from Ptrc RiboJ in pEEK3	Km	This study
Ts3N	Treponema socranskii PAL expressed from Ptrc RiboJ in pEEK3	Km	This study
Ts3N-H87L	Treponema socranskii PAL with mutation H87L expressed from Ptrc RiboJ in pEEK3	Km	This study
EvCΔslr1573	Control strain of Synechocystis WT harboring pEEK* plasmid for Km resistance with knocked-out slr1573	Km, Cm	This study
AtCΔslr1573	Arabidopsis thaliana PAL expressed from Ptrc RBS* in pEEK with knocked-out slr1573	Km, Cm	This study
AtNΔslr1573	Arabidopsis thaliana PAL expressed from Ptrc RBS* in pEEKN with knocked-out slr1573	Km, Cm	This study
TsCΔslr1573	Treponema socranskii PAL expressed from Ptrc RBS* in pEEK with knocked-out slr1573	Km, Cm	This study
TsNΔslr1573	Treponema socranskii PAL expressed from Ptrc RBS* in pEEKN with knocked-out slr1573	Km, Cm	This study