Fig. 6. Tuft cell-derived CysLTs in concert with IL-25 contribute to Alternaria-induced early eosinophilia and systemic immunity.

(A) Ltc4s^fl/fl, Pou2f3^−/− and Chat^CreLtc4s^fl/fl mice were given a single intranasal dose of Alternaria and were evaluated 24 hours after the challenge. (B) Bronchoalveolar lavage cells were counted, and eosinophils and neutrophils were evaluated using Diff-Quik stain. (C) Mediastinal lymph node (MLN) numbers of CD45⁺ cells, CD4⁺ and CD8⁺ T cells and B cells were evaluated by FACS. (D) Mice were given two intraperitoneal injections of an anti-IL-25 antibody 2 hours before and 22 hours after a single intranasal Alternaria inhalation and were evaluated by FACS 48 hours later (E) Mediastinal lymph node (MLN) numbers of CD45⁺ cells, CD4⁺ and CD8⁺ T cells, B cells and DCs were evaluated by FACS. (F) Lung CD45⁺ cells, eosinophils, CD4⁺ T cells, B cells and Thy1⁺ ILC2s were assessed by FACS. Data are means ± SEM pooled from ≥ 2 independent experiments, each dot is a separate mouse, p values from Mann Whitney U-test p values < 0.05 are indicated.