Figure 3.

Biosynthesis of docosanoids by human and mouse eosinophils. EOS suspensions (5 × 10⁶ cells/mL, 37 °C) were incubated with DMSO or increasing concentrations of DHA for 15 min. Incubations were stopped by adding one volume of cold (−20 °C) MeOH containing the internal standards. Samples were then processed and analyzed for docosanoids by LC-MS/MS as described in Materials and Methods. (A,B) Results are the mean (± SEM) of 5 independent experiments using human eosinophils from asthmatic donors. (C,D) Results are the mean (±SEM) of 7 independent experiments using bone marrow-derived mouse eosinophils.