Skip to main content
. 2022 Jan 5;14(1):259. doi: 10.3390/cancers14010259

Figure 5.

Figure 5

LOX expression in HL lymph node stroma and HL cells. Digital images of lymph node sections of the HL 23014-18 were acquired using the Aperio ScanScope Slide program of the AperioAT2 Scanner at 20× magnification. Panel (A): Subpanel a: Mallory trichrome images. Subpanel (b): IHC with the anti-TG2 mAb followed by GAR and DAB. Subpanel c: IHC with the anti-LOX rabbit ab174316 mAb. Panel (B): Layers of each virtual Mallory slide (a) or TG2 (b) or LOX (c) IHC stained slides deconvoluted with the automated image analysis algorithm (Aperio version 9.1). Insets in A and B: images of the whole section with the square indicating the region of enlargement. Panel (C): digital images of sections double stained with the anti-CD30 BerH2 mAb (subpanels (a,b)) or the anti-MUM-1 mAb (subpanel (c)) followed by the specific GAM and DAB (brown) and anti-LOX rabbit ab174316 mAb followed by GAR and Via Green Chromogen (green). Subpanels (d,e): enlargement of the white squares in subpanels (Ca,Cb).