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. 2022 Jan 3;5(1):8–19. doi: 10.1021/acsptsci.1c00182

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© 2022 The Authors. Published by American Chemical Society

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Selection of the optimal donor/acceptor antibody pair. (A) Illustration of the HTRF assay for SARS-CoV-2 NP showing the Eu donor-conjugated primary MAb and DL650 acceptor-conjugated primary MAb detecting SARS-CoV-2 and enabling FRET. (B) Five MAbs specific for SARS-CoV-2 NP labeled with europium (donor Ab) or DyLight650 (acceptor Ab). The labeled MAbs were then tested in HTRF assay, in triplicate wells using a cross-matrix assay format. The presented data are median TR-FRET counts (acceptor fluorescence/donor fluorescence X 10,000). The MAb pair (R001-Eu/1C7-DyLight650) showed the highest specific signal and was selected for further assay development.