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Detecting SARS-CoV-2 NP from Vero E6 TCS and cell lysate. TCS and cell lysate were collected from Vero E6 cells after infection with SARS-CoV-2 USA-WA1/2020 strain for 24 or 48 h. The TR-FRET ratio from TCS (A,C) and cell lysates (B,D) after incubating with reagents for 1 h at RT or O/N at 4 °C. TCS was diluted 1:3, and cell lysate was first diluted 15-fold followed by 1:3 dilutions. N = 3 wells in a 384-well plate. Error bars indicate S.D.
