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. 2022 Jan 3;5(1):8–19. doi: 10.1021/acsptsci.1c00182

Figure 5.

Figure 5

HTRF assay detects NP produced by live virus infection of Vero E6. The TR-FRET ratio for SARS-CoV-2 NP detected in Vero E6 infected with the SARS-CoV-2 USA-WA1/2020 strain at different MOIs starting at 0.25 and diluted 1:3 based on a reverse time course protocol for (A) 0 h (B), 12 h (C), 24 h (D), or 48 h (E). The standard curve (F) with assay conditions equal to the 48h time point. Plates were incubated with NP HTRF reagents for 1 h at RT or O/N at 4 °C. N = 3 wells in a half-area 96-well plate. Error bars indicate S.D.