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. 2022 Jan 3;5(1):8–19. doi: 10.1021/acsptsci.1c00182

Figure 7.

Figure 7

NP HTRF assay detects Beta, Gamma, and Epsilon VoC. The TR-FRET ratio for SARS-CoV-2 NP in Vero E6 infected with the USA-WA1/2020 Beta, Gamma, and Epsilon strains using protocol (A) at MOIs starting at 0.25 and diluted 1:3 for 0 h (B), 4 h (C), 8 h (D), 12 h (E), and 24 h (F). The standard curve (G) with assay conditions equal to the 24 h time point. Assay completed with a reverse time course protocol; O/N incubation at 4 °C with HTRF reagents (5 nM D/10 nM A). N = 3 wells in a half-area 96-well plate. Error bars indicate S.D.