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. 2022 Jan 6;11:e67301. doi: 10.7554/eLife.67301

Figure 4. IgG1 monoclonal antibodies (mAbs) against protein components bind planktonic bacteria as well as biofilm.

(A) Planktonic bacteria of Wood46 (left) and LAC∆spa∆sbi (right) were grown to exponential phase and incubated with a concentration range of rF1-IgG1 or T1-2-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. (B) Biofilms of Wood46 (left) and LAC∆spa∆sbi (right) were grown for 24 hr and incubated with a concentration range of rF1-IgG1 or T1-2-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of three independent experiments.

Figure 4.

Figure 4—figure supplement 1. Binding of the monoclonal antibody (mAb) panel to stationary phase planktonic cultures.

Figure 4—figure supplement 1.

Planktonic bacteria of Wood46 (A) LAC∆spa∆sbi (B) were grown to stationary phase and incubated with a concentration range of mAbs. mAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. Data represent mean + SD of three independent experiments.