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. Author manuscript; available in PMC: 2022 Nov 5.
Published in final edited form as: J Proteome Res. 2021 Oct 25;20(11):5189–5195. doi: 10.1021/acs.jproteome.1c00708

Figure 3.

Figure 3.

Analysis and validation of the MRM quantification results of GTP-binding proteins. (A) Comparison between the forward and reverse labeling experiments of the WM pair of cell lines; (B) comparison between the forward and reverse labeling experiments of the IGR pair of cell lines; (C) comparison between the average ratios for WM and IGR pairs of cell lines; proteins that are substantially up- and downregulated in both lines of metastatic melanoma cells are colored in red and blue, respectively; and (D) western blot for the validation of expression levels of selected GTP-binding proteins with western blot; “P” and “M” designate primary and metastatic cell lines, respectively.