Figure 5.

MLK3 deficiency reduced ethanol-induced liver injury but not steatosis in mice. C57BL/6 and MLK3^−/− mice were allowed free access to diets with increasing concentrations of ethanol (final concentration 32% of kcal) or pair fed a control diet for 25 days. (A) Enzyme activities of ALT and AST were measured in plasma. (B) Hepatic triglyceride content was measured in whole-liver homogenate. (C) Liver histology was visualized in hematoxylin and eosin (H&E)-stained liver sections using a 10× objective. Values represent means ± SEM, n = 4 pair-fed and 6 EtOH-fed mice. Values with different lower case letters are significantly different from each other, p < 0.05.