Figure 7.

MLK3-deficient mice were protected from chronic ethanol-induced apoptosis. C57BL/6 and MLK3^−/− mice were allowed free access to diets with increasing concentrations of ethanol (final concentration 32% of kcal) or pair fed a control diet for 25 days. Paraffin-embedded livers were deparaffinized followed by (A) TUNEL or (B) M30 staining. Images were acquired using 40× for TUNEL and 20× objectives for M30. (C) TUNEL⁺ cells were counted and expressed as percent positive of total number of cells (detected by DAPI staining, not shown in the image). (D) M30⁺ cells per 20× frame were enumerated. Values represent means ± SEM, n = 4 pair-fed and 6 EtOH-fed mice. Values with different lower case letters are significantly different from each other, p < 0.05.