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. Author manuscript; available in PMC: 2022 Sep 22.
Published in final edited form as: J Vis Exp. 2021 Sep 22;(175):10.3791/62617. doi: 10.3791/62617

Figure 2. Treatment of BCBM organotypic brain slices with radiation.

Figure 2.

(A) Representative images depicting tumor growth in organotypic brain slices derived from mice intracranially injected with MDA-MB-231BR-GFP-Luciferase cells detected via bioluminescence. Tumors are exposed to no irradiation or 6 Gy irradiation (one dose) and allowed to grow for the indicated days. Quantification of tumor growth at indicated day (control. n = 3; 6Gy, n=3). Student’s t-test reported as mean ± SEM. * p < 0.05.(B) Brain slices were imaged for 48 h every 15 min. Representative images of videos of non-irradiated and irradiated samples indicate tumor growth. (C) Slices, as treated in (A), were fixed and assayed for H&E, g-H2AX, DAPI, cleaved-Caspase-3 (CC3), and GFP staining (image magnification 20x, scale bar: 200 μm). (D) Tumor-free mouse brain slices were treated as in (A) and were fixed and assayed for CC3 and DAPI (image magnification 20x, scale bar: 200 μm). Please click here to view a larger version of this figure.