Fig. 2.

MiR-133a targets YES1 by binding to the 3′-UTR of YES1. A YES1, TTYH3, CTBP2, YPEL2, and KCTD20 were selected as potential downstream genes regulated by miR-133a by intersection analysis from 3 databases (miRDB, TargetScan, and microRNA). B Among the 5 potential genes, the YES1 expression level was dramatically increased in drug-resistant ovarian cancer tissues compared with primary ovarian cancer tissues. C The predicted miR-133a binding sequence in the 3’UTR of YES1 and the generation of dual-luciferase reporter plasmids of YES1-Wt and YES1-Mut are shown. D Luciferase activity assays were carried out in A2780 cells cotransfected with miR-133a mimic or miR-133a NC and YES1-Wt or YES1-Mut. For comparisons, Student’s t test was performed; *p < 0.05