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. Author manuscript; available in PMC: 2022 Sep 3.
Published in final edited form as: J Proteome Res. 2021 Aug 12;20(9):4529–4542. doi: 10.1021/acs.jproteome.1c00473

Figure 1. Clickable glutathione approach for detection of glutathionylation.

Figure 1.

Glutathione synthetase mutant (GS M4) uses azido-Ala to synthesize azido-glutathione (γGlu-Cys-azido-Ala, N3-GSH). After incubation of azido-Ala to HL-1 cells expressing GS M4, azido-glutathione forms S-glutathionylation, which can be detected after the click reaction.