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. Author manuscript; available in PMC: 2022 Sep 3.
Published in final edited form as: J Proteome Res. 2021 Aug 12;20(9):4529–4542. doi: 10.1021/acs.jproteome.1c00473

Figure 6. Desmin and BAG3 are glutathionylated upon addition of palmitate during OGD/R.

Figure 6.

(A) Glutathionylation of desmin C332. (B) Glutathionylation of BAG3 C179. HL-1 cells expressing GS M4 were used without transfection. Alternatively, HEK293/GS M4 cells were transfected with wild-type or cysteine mutants of desmin or BAG3. Cells were then subjected to OGD for 8 h, followed by the addition of oxygen, glucose, and palmitate with BSA (OGD/OGF) or oxygen-glucose only (OGD/OG) for 2 h. Glutathionylated proteins were processed for click reaction with biotin-alkyne and pull-downs with streptavidin beads and analyzed by western blotting with individual antibodies for desmin, BAG3, and FLAG. Data are representative of 2 independent experiments. A symbol (►) indicates a non-specific band. Two bands in the FLAG-BAG3 blot appear to result from two BAG3 forms.62