Figure 3.

Quantitative assessment of myelin-related protein densities at 16 days of age. (A) Western blot analysis of MBP, PLP1, HMGCS1, MAG, and MOG protein (blue font) expression in tissue isolated from the PFCWM, CC-Genu, CC-Body, and CC-Splenium of control (C) and germ-free (GF) animals at P16. Loading controls: β-Actin for MBP, GAPDH for PLP1, HMGCS1, MAG, and MOG. (B) Quantification of protein expression normalized to respective loading controls. Individual male and female animals are marked by orange triangles and circles, respectively. Data expressed as mean ± SEM, n = 3 animals/group. No significant differences determined by unpaired student’s t-test or Mann-Whitney U test. PLP1, proteolipid protein; HMGCS1, Hydroxymethylglutaryl-CoA synthase; MAG, myelin-associated glycoprotein; MOG, myelin oligodendrocyte glycoprotein.