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. 2021 Dec 27;15:807170. doi: 10.3389/fncel.2021.807170

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Copyright © 2021 Ahmed, Travis, Díaz-Bahamonde, Porter, Henry, Mykins, Ravipati, Booker, Ju, Ding, Ramesh, Pickrell, Wang, LaConte, Howell, Yuan and Morton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Region-specific reduction oligodendrocyte proliferation in germ-free piglets at P16. (A) Representative confocal images showing all cells (DAPI+, blue), OLs (Olig2+, red), proliferating cells (Ki67+, green), and proliferating OLs (Olig2+Ki67+, yellow) and merged images in P16 control and germ-free (GF) animals. Scale bars, 50 μm. Quantification of the number of Ki67+ cells (B) and Olig2+Ki67+ proliferating OLs (C). Individual male animals are marked by yellow triangles and female animals are marked by yellow circles. Data expressed as mean ± SEM, n = 3–5 animals/group. **p < 0.0001, two-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001, unpaired student’s t-test.