Figure 1.
CircZNF609 interacts with several mRNAs in rhabdomyosarcoma cells
(A) Cartoon depicting the circZNF609 pull-down strategy. AMT, 4′-aminomethyl-4,5′,8-trimethylpsoralen; BSJ, back-splicing junction.
(B) qRT-PCR showing the enrichment of circZNF609, ZNF609 mRNA, and GAPDH mRNA in the first replicate of the psoralen-crosslinked circZNF609 pull-down (PD) and control LacZ pull-down in RD cells. Data are shown as means of enrichment versus input ± SE of technical triplicates.
(C) Top: normalized RNA-sequencing read densities in ZNF609 locus for Odd, Even, and LacZ pull-down. Bottom: cartoon depicting ZNF609 locus and ZNF609 exon 2 (Ex2)-derived circRNA (blue).
(D) qRT-PCR showing the enrichment of circZNF609 and its candidate mRNA interactors after native circZNF609 pull-down (PD) or control LacZ pull-down. n = 3. Data are shown as means of enrichment versus input ± SD.