REAGENT OR RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat anti-mouse IGF-II	R&D Systems	RRID: AB_2122524
Biotinylated goat anti-mouse IGF-II	R&D Systems	RRID: AB_2122525
Anti-DIG-AP antibody	Roche	RRID: AB_2734716
Goat anti-human IGF2	R&D Systems	RRID: AB_354449
Goat anti-mouse SOD1	R&D Systems	RRID: AB_2239724
Rabbit anti-goat IgG-HRP	Santa Cruz	RRID: AB_656964
Goat anti-GFP	Abcam	RRID: AB_305643
Rabbit anti-CD31	Abcam	RRID: AB_726362
Goat anti-CD31	R&D Systems	RRID: AB_2161028
Rat anti-mouse F4/80	Bio-Rad	RRID: AB_2098196
Chicken anti-MCT1	Merk Millipore	RRID: AB_90565
Rabbit anti-MCT4	Merk Millipore	RRID: AB_2286063
Rabbit anti-laminin	Dako	RRID: AB_2313665
Rabbit anti-pan cytokeratin	Novus Biologicals	RRID: AB_2296857
Rat anti-mouse CD326/Epcam	BD Biosciences	RRID: AB_394370
Donkey anti-goat-AF488	Jackson ImmunoResearch	RRID: AB_2340430
Donkey anti-rabbit-AF594	Jackson ImmunoResearch	RRID: AB_2340619
Goat anti-rabbit, biotinylated	Abcam	RRID: AB_954902
NL557-conjugated donkey anti-goat	R&D Systems	RRID: AB_663766)
Rabbit anti-rat	Bethyl	RRID: AB_10681533
Anti-rabbit HRP	Vector Labs	RRID: AB_2631198
Donkey anti-chicken-AF488	Jackson ImmunoResearch	RRID: AB_2340376
Goat anti-rabbit-AP	Abcam	RRID: AB_954595
Donkey anti-rat-AF594	Thermo Fisher Scientific	RRID: AB_2535795
Rat anti-CD16/32	BioLegend	RRID: AB_1574975
Rat anti-mouse CD41-PE	BioLegend	RRID: AB_2129745
Rat anti-mouse CD31-AF647	BioLegend	RRID: AB_2161029
Rat anti-mouse CD326-AF647	BioLegend	RRID: AB_1134101
Rat anti-mouse CD41-BV421	BioLegend	RRID: AB_10960744
Rat anti-mouse CD117-PE	BioLegend	RRID: AB_313217
Rat anti-mouse Ly-6A/E-BV510	BioLegend	RRID: AB_2561593
Rat anti-lineage cocktail-BV421	BioLegend	RRID: AB_11203535
Rat anti-mouse CD45-V500	BD Biosciences	RRID: AB_10697046
Rat anti-mouse CD34-AF700	Thermo Fisher Scientific	RRID: AB_493998
Rabbit anti IGF2R	Cell Signaling	RRID: AB_2798462
Mouse anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)	Cell Signaling	RRID: AB_331768
Rabbit anti-p44/42 MAPK (Erk1/2)	Cell Signaling	RRID: AB_330744
Rabbit anti-Phospho-Akt (Ser473)	Cell Signaling	RRID: AB_329825
Rabbit anti-Akt	Cell Signaling	RRID: AB_329827
Rabbit anti-HSP90	Cell Signaling	RRID: AB_2233307
Chemicals, peptides, and recombinant proteins
RIPA buffer	Sigma	R0278
Streptavidin Sulpho-TAG	MSD	R32AD-1
Biotinylated lectin	Vector Laboratories	RRID: AB_2314661
pCR2.1-TOPO plasmid	Thermo Fisher Scientific	K450002
Digoxigenin	Roche	11175025910
Blocking reagent	Roche	1096176001
BCIP/NBT mix	Promega	S3771
Pierce BCA Assay Protein kit	Thermo Fisher Scientific	23225
12-well NuPAGE Novex 4–12% Bis-Tris precast gels	Thermo Fisher Scientific	NP0322BOX
Novex Sharp protein standard	Invitrogen	LC5800
iBlot Transfer Stacks	Invitrogen	IB3010-01
Clarity ECL Western Blotting Substrate	Bio-Rad	1705060
Stripping buffer	Thermo Fisher Scientific	21059
DAPI	Sigma	D9542
RNeasy Plus Micro Kit	Qiagen	74034
RNeasy Plus Mini Kit	Qiagen	74134
RNeasy Midi Kits	Qiagen	75144
RNA 6000 Pico Kit	Agilent	5067-1513
RNA 6000 Nano Kit	Agilent	5067-1511
RevertAid RT Reverse Transcription Kit	Thermo Fisher Scientific	K1622
SYBR Green JumpStart Taq Ready Mix	Sigma	S4438
RBC lysis buffer	BioLegend	420301
Staining buffer	BioLegend	420201
7-Aminoactinomycin	Invitrogen	A1310
5-ethynyl-2′-deoxyuridine	Thermo Fisher Scientific	A10044
Red LIVE/DEAD Fixable Dead Cell Stain	Thermo Fisher Scientific	L23102
Collagenase type I	Sigma	SCR103
Endothelial mitogens	Sigma	E2759
Serum replacement media	Sigma	S0638
Picropodophyllotoxin	Sigma	T9576
Accutase	Sigma	A6964
Angiogenesis μ-Slides	Ibidi	81506
Matrigel	BD Biosciences	354234
Critical commercial assays
Mouse IGF-II DuoSet ELISA kit	R&D Systems	RRID:AB_2884002
In Situ Cell Death Detection Kit, TMR red	Sigma	012156792910
TUNEL Assay Kit – BrdU-Red	Abcam	ab66110
Click-iT EdU Alexa Fluor 488 Imaging Kit	Invitrogen	C10337
Mouse Gene 1.0 ST Array	Affymetrix	901171
Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit	Thermo Fisher Scientific	C10420
Mouse recombinant IGF2	R&D Systems	792-MG-050
Human IGF2Leu27	GroPep	TU100
Complete endothelial growth medium	Cell Biologics	M1168
Basal endothelial growth medium	Cell Biologics	M1168b
Stealth siRNA for Igf2r	Thermo Fisher Scientific	1320003
Stealth scrambled siRNA	Thermo Fisher Scientific	12935100
Lipofectamine RNAiMax transfection reagent	Thermo Fisher Scientific	13778075
Deposited data
Processed gene expression data from expression microarray and RNA-seq obtained in the Igf2EpiKO model	This paper	GSE125434
Processed gene expression data from RNA-seq obtained in the Igf2ECKO model	This paper	GSE179549
Experimental models: Cell lines
primary placental microvascular endothelial cells isolated from C57BL/6J mice	Cell Biologics	C57-6056
Experimental models: Organisms/strains
Mouse Igf2fl/fl	Hammerle et al., 2020	N/A
Mouse Meox2Cre	Tallquist and Soriano, 2000	The Jackson Laboratory (Stock No: 003755)
Mouse TekCre	Kisanuki et al., 2001	The Jackson Laboratory (Stock No: 008863)
Mouse Cyp19Cre	Wenzel and Leone, 2007	N/A
Mouse CMVCre	Schwenk et al., 1995	The Jackson Laboratory (Stock No: 006054)
Mouse VaviCre	de Boer et al., 2003	The Jackson Laboratory (Stock No: 008610)
Mouse Rosa26flSTOPflYFP	Srinivas et al., 2001	The Jackson Laboratory (Stock No: 006148)
Mouse Ai9(RCL-tdT)	Madisen et al., 2010	The Jackson Laboratory (Stock No: 007909)
Mouse H19-DMDfl/fl	Srivastava et al., 2000	N/A
Mouse Igf1rfl/fl	Dietrich et al., 2000	The Jackson Laboratory (Stock No: 012251)
Mouse Igf2rfl/fl	Wylie et al., 2003	N/A
Mouse: C57BL/6J	Charles River Laboratories	Charles River Laboratories (Strain Code 632)
Oligonucleotides
Primers used for genotyping or qRT-PCR, see Table S6	This paper	Sigma Aldrich (Merck)
Software and algorithms
GraphPad Prism 8 software	GraphPad	https://www.graphpad.com/
R3.3	R Foundation	https://www.r-project.org/
MSD Workbench Software	MSD	https://www.mesoscale.com
ImageLab	Bio-Rad	https://www.bio-rad.com
NewCAST	Visiopharm	https://visiopharm.com
ZEN 2009	Carl Zeiss	https://www.zeiss.com/microscopy
Volocity 6.3	Improvision	https://www.perkinelmer.com
HALO	PerkinElmer	https://www.perkinelmer.com
GeneSpring GX 12.1	Agilent	https://www.agilent.com
Ingenuity Pathway Analysis	Qiagen	https://digitalinsights.qiagen.com
FlowJo v.10	TreeStar	https://www.flowjo.com
Angiogenesis Analyzer	Schneider et al., 2012	https://imagej.nih.gov/ij/
TopHat 2.0.11	Kim et al., 2013	https://ccb.jhu.edu/software/tophat
Cufflinks 2.2.1	Trapnell et al., 2010	https://github.com/cole-trapnell-lab/cufflinks
DAVID v6.8	LHRI	https://david.ncifcrf.gov/
REViGO	Supek et al., 2011	http://revigo.irb.hr
EPD	Swiss Institute of Bioinformatics	https://epd.vital-it.ch/index.php
AME v4.12.0	MEME Suite	http://meme-suite.org