Figure 7.

Evaluation of Nanobody expression. Escherichia coli WK6 strain containing pHEN6C vector and expressing Nb 3hCTL55 were induced by 1 M IPTG. After periplasmic extraction with osmotic shock, the quality of the purified and concentrated protein was evaluated with %15 SDS-PAGE (A) and western blotting (B). (A) 1; Purified and concentrated protein 2; periplasmic extraction sample after inducing with IPTG 3; Before inducing with IPTG M; protein marker, (B) 1; a single band of Nb M; protein marker