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. 2022 Jan 7;14:123–133. doi: 10.2147/CMAR.S327621

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© 2022 Dai et al.

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Exosomes derived from hypoxic breast cancer cells promoted the proliferation, migration and angiogenesis of HUVECs. Exosomes were isolated from breast cancer cells under hypoxia or normoxia. Then, HUVECs were co-cultured with indicated exosomes. (A) CCK-8 assay was applied to measure cell viability. (B) Wound healing assay was used to detect cell migration. (C) Transwell migration assay was used to detect cell migration. (D) Tube formation assay was applied to measure angiogenesis. **P<0.01; n = 3.