Fig. 4.

DHHC3 ablation and chemotherapeutic drug effects on oxidative stress. a CellRox fluorescence is used to assess oxidative stress in cells (MDA-MB-231, top panel; BT-549, middle panel; PC3, bottom panel) that were DHHC3-ablated (D3 KD) or not ablated (Cont KD) and treated with either DMSO or camptothecin (CAMP) for 24 h in complete media with 5% serum. b Cells were treated as in panel A, except that oxaliplatin at a concentration of 10 µg/ml for 24 h in media with 5% serum was used. c Cells were treated as in a, except that 5-fluorouracil at a concentration of 7.5 µg/ml for 24 h in complete media with 5% serum was used