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. 2021 Dec 29;17(12):e1009756. doi: 10.1371/journal.pcbi.1009756

Table 2. Strains and plasmids used in this study.

Strain Genotype Reference
BW25113 F-, Δ(araD-araB)567 Δ(rhaD-rhaB)568 ΔlacZ4787 (::rrnB-3) hsdR514 rph-1 [21]
JW5100-1 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔtolB789::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514 [22]
RKCK5 tolA::GFP(ΔMunI)-tolA [18]
RKCK8 pal::pal-GGGGS-mCherry(ΔAgeI)(ΔEcoRI)-kan [5]
RKCK10 ΔtolA pal::pal-GGGGS-mCherry(ΔAgeI)(ΔEcoRI)-kan [5]
JSCK9 tolB::tolB-GGGGS-mCherry(ΔAgeI)(ΔEcoRI) This study
Plasmid Genotype Reference
pBAD24 [23]
pUC19 [24]
pMDISI [20]
pREDTKI [20]
pNP4 pBR322-GFP-tolA [7]
pJS50 pUC19-641 bp N-terminus tolB-GGGGS-mCherry-I-SceI-spcR-I-SceI-50 bp N-terminus mCherry-300 bp downstream tolB This study
pJS61 pBAD24-GFP-t-tolA This study