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. 2022 Jan 7;17:91–104. doi: 10.2147/IJN.S344491

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© 2022 Lin et al.

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Cellular uptake efficiency of Cy5-labeled PαLA-MP NPs. (A and B) Confocal laser scanning microscopy (CLSM) images of (A) BV2 microglial and (B) PC12 cells after incubation with Cy5-PαLA-MP NPs for 1, 2, or 4 h. (C and D) Semiquantitative analysis of Cy5 fluorescence intensity in (C) BV2 and (D) PC12 cells from CLSM images. (E and F) Flow cytometry analysis (FCA) results of Cy5 uptake into (E) BV2 and (F) PC12 cells incubated with Cy5-PαLA-MP NPs for 1, 2, or 4 h. (G and H) Cy5 fluorescence intensity in (G) BV2 and (H) PC12 cells measured using semiquantitative FCA.