| Strain, strain background(Mus musculus, female, C57BL/6) |
UM-HET3 |
Dr. Miller lab |
|
Harrison et al., 2021 (cited in the paper) |
| Strain, strain background(M. musculus, female, C57BL/6) |
Wild type |
Jackson Laboratories |
|
|
| Antibody |
Rabbit polyclonal anti-HPRT antibody |
ProteinTech |
150-59-1-AP |
WB (1:5000) |
| Antibody |
Rabbit polyclonal anti-SDHA antibody |
Invitrogen |
45-920-0 |
WB (1:1000) |
| Antibody |
Rabbit polyclonal anti-FTN antibody |
Sigma-Ardrich |
F5012 |
WB (1:1000) |
| Antibody |
Rabbit polyclonal anti-IRP2 antibody |
Sigma-Ardrich |
SAB2101174 |
WB (1:500) |
| Antibody |
Rabbit polyclonal anti-TFR1 antibody |
ProteinTech |
100-84-2-AP |
WB (1:1000) |
| Antibody |
Rabbit polyclonal anti-FPN1 antibody |
Novus Biologicals |
NBP1-21502 |
WB (1:1000) |
| Antibody |
Rabbit monoclonal anti-Hepcidin antibody |
Abcam |
190775 |
WB (1:200)IH (1:20) |
| Antibody |
Mouse polyclonal anti-ubiquitin antibody |
Cell Signaling Technologies |
3933S |
WB (1:1000) |
| Antibody |
Mouse monoclonal anti-GAPDH antibody |
ProteinTech |
60004-1-Ig |
WB (1:5000) |
| Antibody |
Rabbit polyclonal anti-α-Tubulin antibody |
ProteinTech |
66031-1-Ig |
WB (1:5000) |
| Antibody |
HRP-conjugated donkey polyclonal anti-rabbit IgG antibody |
Jackson ImmunoResearch |
711-035-152 |
WB (1:5000) |
| Antibody |
HRP-conjugated donkey polyclonal anti-mouse IgG antibody |
Jackson ImmunoResearch |
715-035-150 |
WB (1:5000) |
| Antibody |
Alexa Fluor 594 goat polyclonal anti-rabbit IgG |
Jackson ImmunoResearch |
111-585-144 |
IH (1:200) |
| Chemical compound, drug |
3-(2-Pyridyl)–5,6-di(2-furyl)–1,2,4-triazine-5′,5″-disulfonic acid disodium salt(Ferrozine) |
Sigma-Aldrich |
82940 |
|
| Chemical compound, drug |
Trichloroacetic acid |
Sigma-Aldrich |
T6399 |
|
| Chemical compound, drug |
Thioglycolic acid |
Sigma-Aldrich |
T3758 |
|
| Chemical compound, drug |
Hemin |
Sigma-Aldrich |
H9039 |
|
| Chemical compound, drug |
RNA-STAT60 |
Teltest |
Cs-502 |
|
| Chemical compound, drug |
Glycogen |
Life Technologies |
AM9510 |
|
| Chemical compound, drug |
Paraformaldehyde |
Thermo Fisher Scientific |
AC416780250 |
|
| Chemical compound, drug |
RIPA Buffer |
Thermo Fisher Scientific |
89901 |
|
| Commercial assay or kit |
ProteaseArrest Protease Inhibitor |
G-Biosciences |
786-437 |
|
| Commercial assay or kit |
qScript cDNA Synthesis Kit |
Quanta |
95047-500 |
|
| Commercial assay or kit |
PerfeCTa SYBR Green FastMix |
Quanta |
95074-05K |
|
| Commercial assay or kit |
SuperSignal West Pico PLUS Chemiluminescent Substrate |
Pierce |
34579 |
|
| Commercial assay or kit |
Dynabeads Protein G for Immunoprecipitation |
Invitrogen |
10003D |
|
| Commercial assay or kit |
BCA Protein Assay Kit |
Pierce |
23225 |
|
| Commercial assay or kit |
Mitochondria Isolation Kit for Tissue |
Pierce |
89801 |
|
| Commercial assay or kit |
Aconitase Activity Assay Kit |
Abcam |
Ab109712 |
|
| Commercial assay or kit |
OTC compound |
Sakura Finetek |
4583 |
|
| Sequence-based reagent |
Primers for qRT-PCR |
This manuscript |
N/A |
Included in the next table |
| Software, algorithm |
GraphPad Prism |
GraphPad |
Version 9 |
|
| Software, algorithm |
ImageJ |
NIH |
1.53c |
|
