Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | ICR wild type | SLC Japan | RRID:MGI:5462094 | |
| Genetic reagent (M. musculus) | C57BL/6N wild type | Leibniz Research Institute for Molecular Pharmacology | RRID:MGI:5651595 | |
| Genetic reagent (M. musculus) | Ap2m1lox/lox × inducible CAG-Cre | Haucke Lab Kononenko et al., 2014 |
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| Recombinant DNA reagent | SEP-tagged VGLUT1 | Takamori Lab Mori et al., 2021 |
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| Recombinant DNA reagent | SEP-tagged Syp | L. Lagnado Granseth et al., 2006 |
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| Recombinant DNA reagent | SEP-tagged Syp | Takamori Lab Egashira et al., 2015 |
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| Recombinant DNA reagent | SEP-tagged Syb2 | S. Kawaguchi | ||
| Recombinant DNA reagent | pLenti6PW-STB | Y. Fukazawa Hioki et al., 2009 |
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| Recombinant DNA reagent | SEP-tagged Syt1 | J. Klingauf Wienisch and Klingauf, 2006 |
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| Recombinant DNA reagent | SEP-tagged Syb2 | J. Klingauf Wienisch and Klingauf, 2006 |
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| Recombinant DNA reagent | SEP-tagged SV2A | E.R. Chapman Kwon and Chapman, 2011 |
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| Recombinant DNA reagent | SEP-tagged VGLUT1 | R. Edwards and S. Voglmaier Voglmaier et al., 2006 |
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| Recombinant DNA reagent | SEP-tagged VGAT | S. Voglmaier Santos et al., 2013 |
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| Recombinant DNA reagent | f(U6)sNLS-RFPw msClathrin scrambled | C. Rosenmund Watanabe et al., 2014 |
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| Recombinant DNA reagent | f(U6)sNLS-RFPw msClathrin shRNA | C. Rosenmund Watanabe et al., 2014 |
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| Recombinant DNA reagent | pLV-shmCHC-hPGK-mCherry | VectorBuilder | ||
| Recombinant DNA reagent | AP2mu-IRES-mRFP in-AAV-HBA-EWB | Haucke Lab López-Hernández et al., 2020 |
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| Chemical compound, drug | Pitstop2 | Haucke Lab von Kleist et al., 2011 |
30μM | |
| Chemical compound, drug | Folimycin | Sigma | Cat# C9705 | 67nM |
| Chemical compound, drug | EZ-Link Sulfo-NHS-LC-Biotin | Thermo Fisher Scientific | Cat# 21,335 | 0.5mg/ml |
| Chemical compound, drug | Streptavidin agarose | Millipore | Cat# 69,203 | 100μl |
| Chemical compound, drug | GST-bind resin | Cytiva | Cat# 17075601 | 120μl |
| Chemical compound, drug | Transferrin 647 | Life Technologies | Cat# T23366 | 25μg/ml |
| Chemical compound, drug | Tamoxifen ((Z)-4-hydroxytamoxifen) | Sigma | Cat# H7904 | 0.1μM |
| Chemical compound, drug | AraC | Sigma | Cat# C6645 | 4μM |
| Chemical compound, drug | Uridine | Sigma | Cat# U3750 | 100μM |
| Chemical compound, drug | Uridine | Sigma | Cat# U3003 | 100μM |
| Chemical compound, drug | FUDR | Sigma | Cat# F0503 | 40μM |
| Commercial assay or kit | ProFection Mammalian Transfection System – Calcium Phosphate | Promega | Cat# E1200 | |
| Commercial assay or kit | CalPhos Mammalian Transfection Kit | Takara | Cat# 631,312 | |
| Antibody | AP-2 α-adaptin(mouse monoclonal) | Haucke Lab | AP6 | IF: 1:100 |
| Antibody | AP-2 α-adaptin(mouse monoclonal) | Santa Cruz | Cat# sc-55497RRID:AB_2056344 | WB: 1:100 |
| Antibody | γ-Adaptin 1(mouse monoclonal) | BD Biosciences | Cat# 610,386RRID:AB_397769 | WB: 1:500 |
| Antibody | σ-Adaptin 3(mouse monoclonal) | Haucke Lab | SA4 | WB: 1:250 |
| Antibody | β-Actin(mouse monoclonal) | Sigma-Aldrich | Cat# A-5441RRID:AB_476744 | WB: 1:5000 |
| Antibody | GAPDH(mouse monoclonal) | Sigma-Aldrich | Cat# G8795RRID:AB_1078991 | WB: 1:5000 |
| Antibody | N-cadherin(mouse monoclonal) | BD Biosciences | Cat# 610,920RRID:AB_610920 | WB: 1:1000 |
| Antibody | CHC(mouse monoclonal) | Haucke lab | TD1 | WB: 1:500 |
| Antibody | CHC(rabbit polyclonal) | Abcam | Cat# ab21679RRID:AB_ 2083165 | IF: 1:1000 |
| Antibody | Synaptophysin(mouse monoclonal) | R. Jahn | Cl 7.2 | IF: 1:1000 |
| Antibody | MAP-2(guinea pig polyclonal) | Synaptic Systems | Cat# 188 004RRID:AB_2138181 | IF: 1:400 |
| Antibody | Synapsin(mouse monoclonal) | Synaptic Systems | Cat# 106 001RRID:AB_887805 | IF: 1:400 |
| Antibody | Synapsin(guinea pig polyclonal) | Synaptic Systems | Cat# 106 004RRID:AB_1106784 | IF: 1:400 |
| Antibody | VGLUT1(guinea pig polyclonal) | Synaptic Systems | Cat# 135 304RRID:AB_887878 | IF: 1:300WB: 1:500 |
| Antibody | Synaptotagmin 1(rabbit polyclonal) | Synaptic Systems | Cat# 105 102RRID:AB_887835 | IF: 1:100 |
| Antibody | Synaptotagmin 1(mouse monoclonal) | Synaptic Systems | Cat# 105 011RRID:AB_887832 | IF: 1:250WB: 1:500 |
| Antibody | VGAT Oyster 488(rabbit polyclonal) | Synaptic Systems | Cat# 131 103C2RRID:AB_10640329 | IF: 1:100 |
| Antibody | VGAT Oyster 568(rabbit polyclonal) | Synaptic Systems | Cat# 131 103C3RRID:AB_887867 | IF: 1:100 |
| Antibody | VGAT(rabbit polyclonal) | Synaptic Systems | Cat# 131 013RRID:AB_2189938 | IF: 1:300 |
| Antibody | VGAT(guinea pig polyclonal) | Synaptic Systems | Cat# 131 004RRID:AB_887873 | WB: 1:500 |
| Antibody | VGAT(rabbit polyclonal) | Synaptic Systems | Cat# 131 103CpHRRID:AB_2189809 | Live imaging:1:120 |
| Antibody | SV2(rabbit polyclonal) | Abcam | Cat# ab32942RRID:AB_778192 | WB: 1:500 |
| Antibody | Peroxidase-AffiniPure Anti-Rabbit IgG (H + L)(goat polyclonal) | Jackson ImmunoResearch Labs | Cat# 111-035-003RRID:AB_2313567 | WB: 1:10,000 |
| Antibody | Peroxidase-AffiniPure Anti-Mouse IgG (H + L)(goat polyclonal) | Jackson ImmunoResearch Labs | Cat# 115-035-003RRID:AB_10015289 | WB: 1:10,000 |
| Antibody | Peroxidase-AffiniPure Anti-guineapig IgG (H + L)(goat polyclonal) | Jackson ImmunoResearch Labs | Cat# 106-035-003RRID:AB_2337402 | WB: 1:10,000 |
| Antibody | Mouse IgG HRP Linked F(ab′)2 Fragment(sheep monoclonal) | Cytiva | Cat# GENA9310RRID:AB_772193 | WB: 1:10,000 |
| Antibody | Anti-mouse IgG, IRDye 800CW Conjugated(goat polyclonal) | LI-COR Biosciences | Cat# 926-32210RRID:AB_621842 | WB: 1:10,000 |
| Antibody | IRDye 680RD anti-mouse IgG (H + L)(goat polyclonal) | LI-COR Biosciences | Cat# 925-68070RRID:AB_2651128 | WB: 1:10,000 |
| Antibody | IRDye 680RD anti-rabbit IgG (H + L)(goat polyclonal) | LI-COR Biosciences | Cat# 926-68071RRID:AB_10956166 | WB: 1:10,000 |
| Antibody | Anti-rabbit IgG, IRDye 800CW Conjugated(goat polyclonal) | LI-COR Biosciences | Cat# 926-32211RRID:AB_621843 | WB: 1:10,000 |
| Antibody | Anti-guinea pig IgG Alexa Fluor 647(goat polyclonal) | Thermo Fisher Scientific | Cat# A-21450RRID:AB_141882 | IF: 1:500 |
| Antibody | Anti-guinea pig IgG Alexa Fluor 488(donkey polyclonal) | Jackson ImmunoResearch Labs | Cat# 706-545-148RRID:AB_141954 | IF: 1:500 |
| Antibody | Anti-guinea pig IgG Alexa Fluor 568(goat polyclonal) | Thermo Fisher Scientific | Cat# A-11075RRID:AB_141954 | IF: 1:500 |
| Antibody | Anti-mouse IgG Alexa Fluor 568(goat polyclonal) | Thermo Fisher Scientific | Cat# A-11004RRID:AB_2534072 | IF: 1:500 |
| Antibody | Anti-rabbit IgG Alexa Fluor 647(goat polyclonal) | Thermo Fisher Scientific | Cat# A-21245RRID:AB_2535813 | IF: 1:500 and 1:1000 |
| Antibody | Anti-rabbit IgG Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11008RRID:AB_143165 | IF: 1:500 |
| Antibody | Anti-mouse IgG Alexa Fluor 488(goat polyclonal) | Thermo Fisher Scientific | Cat# A-11029RRID:AB_2534088 | IF: 1:500 and 1:1000 |
| Software and algorithms | Image J | Open Source Software | https://imagej.net/Welcome | |
| Software and algorithms | Prism v.8 | GraphPad | RRID: SCR_002798 | |
| Software and algorithms | Python | Programming Language | RRID: SCR_008394 |