| Strain, strain background (Mycobacterium tuberculosis) |
M. tuberculosis
|
Laleh Majlessi, Institut Pasteur, Paris |
H37Rv |
Animal passaged |
| Strain, strain background (M. tuberculosis) |
M. tuberculosis DsRed |
Marino et al., 2015
|
H37Rv DsRed |
Animal passaged |
| Strain, strain background (Mycobacterium bovis) |
BCG Pasteur |
Roland Brosch, Institut Pasteur, Paris |
1173P2 |
|
| Strain, strain background (Mus musculus), male |
C57BL/6J |
Charles River Laboratories |
JAX stock no: 000664;RRID: IMSR_JAX:000664
|
|
| Cell line (Homo sapiens) |
THP-1 |
ATCC |
ATCC TIB-202;RRID:CVCL_0006
|
|
| Strain, strain background (Escherichia coli)
|
HB101 |
Promega |
Cat# L2011 |
Recombinant cloning and subcloning strain |
| Gene (M. tuberculosis) |
lucA
|
ATCC27294 |
Rv3723
|
|
| Gene (M. tuberculosis) |
mce1D
|
ATCC27294 |
Rv0172
|
|
| Gene (M. tuberculosis) |
mceG
|
ATCC27294 |
Rv0655
|
|
| Gene (M. tuberculosis) |
omamB
|
ATCC27294 |
Rv0200
|
|
| Gene (M. tuberculosis) |
yrbE1A
|
ATCC27294 |
Rv0167
|
|
| Gene (M. tuberculosis) |
yrbE2A
|
ATCC27294 |
Rv0587
|
|
| Gene (M. tuberculosis) |
yrbE3A
|
ATCC27294 |
Rv1964
|
|
| Gene (M. tuberculosis) |
yrbE4A
|
ATCC27294 |
Rv3501
|
|
| Genetic reagent (M. tuberculosis) |
ΔlucA
|
This study, available from the corresponding author |
ΔlucA::km
|
Chromosomal deletion of Rv3723 (lucA) and insertion of an antibiotic resistance marker by double crossover recombination |
| Genetic reagent (M. tuberculosis) |
Δmce1D
|
This study, available from the corresponding author |
Δmce1D::km
|
Chromosomal deletion of Rv0172 (mce1D) and insertion of an antibiotic resistance marker by double crossover recombination |
| Genetic reagent (M. tuberculosis) |
ΔmceG
|
This study, available from the corresponding author |
ΔmceG::km
|
Chromosomal deletion of Rv0655 (mceG) and insertion of an antibiotic resistance marker by double crossover recombination |
| Genetic reagent (M. tuberculosis) |
ΔomamB
|
This study, available from the corresponding author |
ΔomamB::km
|
Chromosomal deletion of Rv0200 (omamB) and insertion of an antibiotic resistance marker by double crossover recombination |
| Genetic reagent (M. tuberculosis) |
ΔyrbE1A
|
This study, available from the corresponding author |
ΔyrbE1A::km
|
Chromosomal deletion of Rv0167 (yrbE1A) and insertion of an antibiotic resistance marker by double crossover recombination |
| Genetic reagent (M. tuberculosis) |
ΔyrbE2A
|
This study, available from the corresponding author |
ΔyrbE2A::km
|
Chromosomal deletion of Rv0587 (yrbE2A) and insertion of an antibiotic resistance marker by double crossover recombination |
| Genetic reagent (M. tuberculosis) |
ΔyrbE3A
|
This study, available from the corresponding author |
ΔyrbE3A::km
|
Chromosomal deletion of Rv1964 (yrbE3A) and insertion of an antibiotic resistance marker by double crossover recombination |
| Genetic reagent (M. tuberculosis) |
ΔyrbE4A
|
This study, available from the corresponding author |
ΔyrbE4A::km
|
Chromosomal deletion of Rv3501 (yrbE4A) and insertion of an antibiotic resistance marker by double crossover recombination |
| Genetic reagent (M. tuberculosis) |
Δmce1D Comp
|
This study, available from the corresponding author |
Δmce1D::km::yrbE1 to rv0178
|
WT copy of the yrbE1 to rv0178 operon integrated at AttB |
| Recombinant DNA reagent |
PX458 (plasmid) |
Addgene(Ran et al., 2013) |
pSpCas9(BB)–2A-GFP (pX458) |
|
| Recombinant DNA reagent |
pET26b (plasmid) |
Sigma-Aldrich |
pET26b(+) - Novagen |
|
| Recombinant DNA reagent |
pJV53H (plasmid) |
This study, available from C. Guilhot |
pJV53H |
pJV53 (van Kessel and Hatfull, 2007) with hygromycin resistance gene |
| Recombinant DNA reagent |
pMV361 (plasmid) |
Stover et al., 1993
|
pMV361 |
AttB integrating M. tuberculosis plasmid |
| Recombinant DNA reagent |
pMVZ621 (plasmid) |
Didier Zerbib, Toulouse Biotechnology Institute, France |
pMVZ621 |
pMV261 carrying a Zeocin resistance gene |
| Recombinant DNA reagent |
pWM430 (plasmid) |
This study |
pWM430 |
pMV361 with insertion of a zeocin cassette between the XbaI and Eco147i restriction sites |
| Recombinant DNA reagent |
pWM431 (plasmid) |
This study |
pWM431 |
pWM430 with a fragment going from gene yrbE1A to gene rv0178 of M. tuberculosis H37Rv |
| Recombinant DNA reagent |
pWM251 (plasmid) |
This study |
pWM251 |
pMIP12 (Le Dantec et al., 2001) carrying the streptomycin resistance cassette from pHP45Ω (Prentki and Krisch, 1984) and the gfp under the control of the pBlaF* promotor |
| Recombinant DNA reagent |
Rv165 (cosmid) |
Brosch et al., 1998
|
Rv165 cosmid |
Cosmid carrying a large fragment of the H37Rv genome covering the Mce1 region. |
| Sequence-based reagent |
Primers used to generate AES and Mtb mutants |
Merck |
PCR primers |
Supplementary file 2
|
| Sequence-based reagent |
|
Eurofins Genomics |
qPCR primers |
Supplementary file 3
|
| Sequence-based reagent |
FADS2_ex2_F |
Eurofins Genomics |
PCR primer |
5′-GCACATTTCCAGTGCCAAGG-3′ |
| Sequence-based reagent |
FADS2_ex2_R |
Eurofins Genomics |
PCR primer |
5′-GGAGAGAGGAGACGCCACTA-3′ |
| Sequence-based reagent |
Guide RNA targeting the exon 2 of FADS2
|
Eurofins Genomics |
Oligonucleotide |
5′-GCACCCTGACCTGGAATTCGT-3′ |
| Transfected construct (M. musculus) |
ON-TARGETplus siRNAs (SMARTpool) |
Dharmacon/Horizon Discoveries |
Non-targeting: D-001810-10Mouse Fads2:L-049816-01 |
|
| Antibody |
Anti-FADS2 (rabbit polyclonal) |
Thermo Fisher Scientific |
PA576611; RRID:AB_2720338
|
(1:1000) dilution |
| Antibody |
Anti-GAPDH (rabbit monoclonal) |
Cell Signaling Technology |
2118; RRID:AB_561053
|
(1:1000) dilution |
| Antibody |
Goat anti-rabbit IgG (HRP conjugate) |
Santa Cruz |
SC-2004;RRID:AB_631746
|
(1:1000) dilution |
| Chemical compound, drug |
SC-26196 |
Cayman Chemical |
10792 |
|
| Chemical compound, drug |
Ultrapure LPS from E. coli
|
Enzo Life Sciences |
ALX-581-013 |
Serotype 055:B5 |
| Chemical compound, drug |
Pam3Csk4 |
Invivogen |
tlrl-pms |
|
| Chemical compound, drug |
Lipofectamine RNAiMAX |
Thermo Fisher Scientific |
13778075 |
|
| Commercial assay or kit |
VenorGeM Advance Mycoplasma detection kit |
Minerva Biolabs |
11-7024 |
|
| Commercial assay or kit |
Human Monocyte Nucleofector Kit |
Lonza |
VPA-1007 |
|
| Commercial assay or kit |
Click-iT Plus Alexa Fluor-647 Picolyl Azide kit |
Thermo Fisher Scientific |
C10643 |
|
| Commercial assay or kit |
Quant-iT dsDNA Assay Kit, broad range |
Thermo Fisher Scientific |
Q33130 |
|
| Commercial assay or kit |
Apoptosis/ Necrosis Assay Kit |
Abcam |
ab176750 |
|
| Commercial assay or kit |
High-Capacity cDNA Reverse Transcription Kit |
Thermo Fisher Scientific |
4368814 |
|
| Commercial assay or kit |
Power SYBR Green PCR Master Mix |
Thermo Fisher Scientific |
4367659 |
|
| Commercial assay or kit |
miRNeasy Mini Kit |
QIAGEN |
217004 |
|
| Commercial assay or kit |
CloneJET PCR Cloning Kit |
Thermo Fisher Scientific |
K1231 |
|
| Commercial assay or kit |
AmpliTaq Gold 360 master mix |
Thermo Fisher Scientific |
4398876 |
|
| Peptide, recombinant protein |
DreamTaq Green polymerase |
Thermo Fisher Scientific |
EP0711 |
|
| Peptide, recombinant protein |
T4 DNA ligase |
Thermo Fisher Scientific |
EL0011 |
|
| Peptide, recombinant protein |
PrimeSTAR GXL DNA Polymerase |
Takara Bio |
R050B |
|
| Software, algorithm |
GraphPad Prism |
GraphPad Prism (https://graphpad.com) |
RRID:SCR_015807
|
|
| Software, algorithm |
Zen Imaging software |
Zeiss |
RRID:SCR_013672
|
|
| Software, algorithm |
Icy opensource platform |
http://www.icy.bioimageanalysis.org
de Chaumont et al., 2012
|
RRID:SCR_010587
|
|
| Software, algorithm |
FlowJo software |
https://www.flowjo.com/
|
RRID:SCR_008520
|
|
| Software, algorithm |
nSolver analysis software |
http://www.nanostring.com/products/nSolver
|
RRID:SCR_003420
|
|
| Other |
4–12% NuPAGE Bis-Tris gels |
Thermo Fisher Scientific |
NP0322BOX |
|
| Other |
iBlot 2 gel transfer Stacks Nitrocellulose system |
Thermo Fisher Scientific |
IB23001 |
|
| Other |
Prolong Diamond Antifade Mountant |
Thermo Fisher Scientific |
P36961 |
|
| Other |
DAPI stain |
Sigma-Aldrich |
D9542 |
Used at 1 µg/mL |
