| Strain, strain background (E. coli) |
ER2267 |
Tawfik lab stock |
|
|
| Strain, strain background (E. coli) |
BW25113 |
Keio collection |
|
|
| Strain, strain background (E. coli) |
BW25113 acrB::Kan
|
Keio collection |
|
|
| Strain, strain background (E. coli) |
Rosetta2 BL21(DE3) pLysS |
Novagen |
|
|
| Strain, strain background (E. coli) |
MRE600 |
ATCC |
|
|
| Gene(Staphylococcus aureus) |
cfr gene |
|
Accession: EF450709.1
|
|
| Gene(Staphylococcus sciuri) |
Sequence upstream cfr gene |
Genscript |
Accession: AJ579365
|
|
| Recombinant DNA reagent |
pZA (plasmid) |
Wellner et al., 2013; Stojković et al., 2016
|
|
|
| Recombinant DNA reagent |
pET28a (plasmid) |
Fujimori lab stock |
|
|
| Recombinant DNA reagent |
pKK3535 (plasmid) |
Fujimori lab stock |
|
|
| Chemical compound, drug |
Anhydrotetracycline hydrochloride |
Sigma-Aldrich |
37919 |
|
| Chemical compound, drug |
Tiamulin |
Wako Chemicals |
328-34002 |
|
| Chemical compound, drug |
Clindamycin |
TCI America |
C2256 |
|
| Chemical compound, drug |
Chloramphenicol |
Allstar Scientific |
480-045 |
|
| Chemical compound, drug |
Linezolid |
Acros |
460592500 |
|
| Chemical compound, drug |
Hygromycin A |
Dr. Kim Lewis |
|
|
| Chemical compound, drug |
Trimethoprim |
Sigma-Aldrich |
92131 |
|
| Chemical compound, drug |
[3H-methyl] S-adenosylmethionine |
PerkinElmer |
NET 155 H001MC |
|
| Chemical compound, drug |
Rifampicin |
Sigma-Aldrich |
R3501 |
|
| Antibody |
Anti-FLAG(mouse monoclonal) |
Sigma-Aldrich |
F3165 |
1:2000 |
| Antibody |
Anti-RNA polymerase beta(rabbit monoclonal) |
Abcam |
ab191598 |
1:2000 |
| Antibody |
Anti-rabbit IgG cross-absorbed DyLight 680 (goat polyclonal) |
Thermo Fisher Scientific |
35567 |
1:10,000 |
| Antibody |
Anti-mouse IgG cross-absorbed IRDye 800CW(goat polyclonal) |
Abcam |
ab216772 |
1:10,000 |
| Antibody |
Anti-GAPDH(mouse monoclonal) |
Abcam |
ab125247 |
1:2000 |
| Sequence-based reagent |
cfr |
Integrated DNA Technologies |
|
For RT-qPCR. The sequence of this oligonucleotide can be found in Supplementary file 1
|
| Sequence-based reagent |
recA |
Integrated DNA Technologies |
|
For RT-qPCR. The sequence of this oligonucleotide can be found in Supplementary file 1
|
| Sequence-based reagent |
luc |
Integrated DNA Technologies |
|
For RT-qPCR. The sequence of this oligonucleotide can be found in Supplementary file 1
|
| Sequence-based reagent |
Luciferase control RNA |
Promega |
L456A |
Control RNA-spike in for polysome analysis |
| Commercial assay or kit |
RNAprotect Bacteria Reagent |
QIAGEN |
76506 |
|
| Commercial assay or kit |
RNeasy Mini Kit |
QIAGEN |
74104 |
|
| Commercial assay or kit |
iScript cDNA Synthesis Kit |
Bio-Rad |
170-8891 |
|
| Commercial assay or kit |
SsoAdvanced Universal SYBRGreen Supermix |
Bio-Rad |
172-5721 |
|
| Peptide, recombinant protein |
RNaseT1 |
Thermo Fisher Scientific |
EN0541 |
|
| Peptide, recombinant protein |
RQ1 RNase-free DNase I |
Promega |
M610A |
|
| Software, algorithm |
Image Lab Software |
Bio-Rad |
|
|
| Software, algorithm |
MotionCor2 |
Zheng et al., 2017
|
|
|
| Software, algorithm |
GCTF |
Zhang, 2016
|
|
|
| Software, algorithm |
CryoSPARC v2.0 |
Punjani et al., 2017
|
|
|
| Software, algorithm |
Relion 3.0 |
Scheres, 2012
|
|
|
| Software, algorithm |
cisTEM |
Grant et al., 2018
|
|
|
| Software, algorithm |
Sharpen3D |
Grant et al., 2018
|
|
|
| Software, algorithm |
PHENIX |
Adams et al., 2010
|
|
|
| Software, algorithm |
eLBOW |
Moriarty et al., 2009
|
|
|
| Software, algorithm |
MolProbity |
Chen et al., 2010
|
|
|
| Software, algorithm |
Pymol Molecular Graphics System |
Schrödinger, LLC |
|
Version 2.4.1 |
