| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal ANTI-FLAG M2 antibody produced in mouse | Sigma-Aldrich/Merck | Cat# F1804 |
| Polyclonal ANTI-HA antibody produced in rabbit | Gift from R. Hegde (MRC-LMB) | N/A |
| Polyclonal ANTI-GFP antibody produced in rabbit | Invitrogen/ThermoFisher Scientific | Cat# A11122 |
| Polyclonal ANTI-ARL13B antibody produced in rabbit | Proteintech | Cat# 17711-1 |
| ANTI-CENT1 – Alexa Fluor 647 | Proteintech/Invitrogen | Cat# 12794-1-AP/ A20186 |
| Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 | Invitrogen/ThermoFisher Scientific | Cat# R37114 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 | Invitrogen/ThermoFisher Scientific | Cat# A10042 |
| Normal Rabbit IgG | Santa Cruz Biotechnology | Cat# sc-2027 |
| Bacterial Strains | ||
| Rosetta (DE3) | Gift from J. Kilmartin (MRC-LMB) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Glycergel Mounting Media | Agilent | Cat# C056330-2 |
| Lipofectamine 3000 | Thermo Fisher Scientific | Cat# L3000001 |
| D-MEM/F-12, supplied,GlutaMAX, sodium carbonate | Thermo Fisher Scientific | Cat# 31331028 |
| D-MEM Glutamax | Thermo Fisher Scientific | Cat# 10566016 |
| Expi293 Expression Medium | Thermo Fisher Scientific | Cat# A1435101 |
| Glutathione sepharose 4B | GE Healthcare Life Sciences | Cat# 17075601 |
| Amylose resin | New England Biolabs | Cat# E8021S |
| anti-FLAG M2 magnetic beads | Sigam-Aldrich/Merck | Cat# M8823 |
| NuPAGE 4-12% Bis-Tris 1.0mm 15-well | ThermoFisher Scientific | Cat# NP0323BOX |
| PEI | Polysciences | Cat# 24765 |
| TTBK21074-1087 peptide | Biomatik | N/A |
| Wizard Classic 2 screen | Rigaku/ Molecular Dimensions | Cat# MD15-W2-T |
| JCSG screen | Qiagen/ NeXtal | Cat# 130920 |
| Wizard Cryo 2 screen | Rigaku/ Molecular Dimensions | Cat# MD15-C2-T |
| Deposited Data | ||
| Crystal structure of Homo sapiens CEP1641-109 in complex with camelid nanobody 10Z | This paper | PDB accession code 7O06 |
| Crystal structure of Homo sapiens CEP1641-109 in complex with camelid nanobody 36Z | This paper | PDB accession code 7O0S |
| Crystal structure of Homo sapiens TTBK21074-1087-CEP1641-109 in complex with camelid nanobody 36Z | This paper | PDB accession code 7O3B |
| NMR structure ensemble of Homo sapiens CEP1641-109 | This paper | PDB accession code 7NWJ |
| NMR data | This paper | BMRB accession code 50793 |
| Crystal structure of the camelid nanobody VHH T9 | Tabares da Rosa et al. (2019) | PDB accession code 5VLV |
| Crystal structure of human EB1 in complex with microtubule Tip localization signal peptide of MACF | Honnappa et al. (2009) | PDB accession code 3GJO |
| Crystal structure of ADP bound TTBK2 kinase domain | Bao et al. (2021) | PDB accession code 6VRF |
| NMR structure of Smurf1 WW domain in complex with a Smad1 derived peptide | Aragón et al. (2011) | PDB accession code 2LAZ |
| NMR structure of YAP WW domain in complex with a Smad1 derived peptide | Aragón et al. (2011) | PDB accession code 2LAW |
| NMR structure of FBP11WW1 domain in complex with a proline-rich peptide | Pires et al. (2005) | PDB accession code 1YWI |
| Crystal structure of FE65 WW domain in complex with hMena peptide | Meiyappan et al. (2007) | PDB accession code 2HO2 |
| Experimental Models: Cell Lines | ||
| hTERT RPE-1 | Gift from C. Morrison (NUI Galway) (Daly et al., 2016) |
N/A |
| CEP164 null hTERT RPE-1 | Gift from C. Morrison (NUI Galway) (Daly et al., 2016) |
N/A |
| Flp-In T-REx 293 | Gift from R. Hegde (MRC-LMB) (Joseph et al., 2018) |
N/A |
| Expi293 | Gift from O. Perisic, R. Williams group (MRC-LMB) | N/A |
| Recombinant DNA | ||
| pGEX6P1-CEP1641-109 (BamH1 and EcoR1 sites) | This paper | N/A |
| pGEX6P1-TTBK21074-1087-CEP1641-109 (BamH1 and EcoR1 sites) | This paper | N/A |
| pGEX6P1-CEP1641-28,50-94 (=ΔHelices)-TEV-HIS6 (BamH1-EcoR1 sites) | This paper | N/A |
| pGEX6P1-camelid nanobody 10Z (BamH1 and EcoR1 sites) | This paper | N/A |
| pGEX6P1-camelid nanobody 36Z (BamH1 and EcoR1 sites) | This paper | N/A |
| pOPTM -TTBK21074-1087 (Nde1 and BamH1 sites) | This paper | N/A |
| pGEX6P1- EB1 (BamH1 and EcoR1 sites) | This paper | N/A |
| pSKB2LNB-EB1-GFP (Nde1 and BamH1 sites) | This paper | N/A |
| pGEX6P1-CEP164N-GFP (BamH1 and EcoR1 sites) | This paper | N/A |
| pcDNA 3.1 derivative – 3xFLAG-TTBK2-3xSTREP (WT or D163A mutant) (BamH1 and EcoR1 sites) | This paper | N/A |
| pcDNA 3.1 derivative – 3xFLAG-CEP164 (WT or Q11P, or R93W, or kinase dead (BamH1 and EcoR1 sites) | This paper | N/A |
| pcDNA 3.1 derivative – 3xHA-TTBK2 (or mutant) (BamH1 and EcoR1 sites) | This paper | N/A |
| Software and Algorithms | ||
| DIALS | (Winter et al., 2018) | https://dials.github.io/ |
| AIMLESS | (Evans and Murshudov, 2013) | http://www.ccp4.ac.uk/download |
| PHASER | (McCoy et al., 2007) | http://www.ccp4.ac.uk/download |
| REFMAC | (Murshudov et al., 2011) | http://www.ccp4.ac.uk/download |
| COOT | (Emsley and Cowtan, 2004) | http://www.ccp4.ac.uk/download |
| PHENIX.REFINE | (Afonine et al., 2012) | http://www.phenix-online.org/download/ |
| MolProbity | (Chen et al., 2010) | http://molprobity.manchester.ac.uk/ |
| The PyMOL Molecular Graphics System | Schrödinger, LLC | Version 2.0 / https://pymol.org/2/#download |
| GraphPad Prism Software | GraphPad Software | Version 6.0 / https://www.graphpad.com/ |
| AUCpreD | (Wang et al., 2016) | http://raptorx.uchicago.edu/StructurePropertyPred/predict/ |
| Multilayered Fusion-based Disorder predictor (MFDp2) | (Mizianty et al., 2013) | http://biomine.cs.vcu.edu/servers/MFDp2/ |
| SPOT-Disorder2 | (Hanson et al., 2019) | https://sparks-lab.org/server/spot-disorder2/ |
| Malvern Panalytical PEAQ software | Malvern | N/A |
| TopSpin | Bruker | Version 3 |
| NMRFAM-SPARKY | (Lee et al., 2015) | https://nmrfam.wisc.edu/nmrfam-sparky-distribution/ |
| XPLOR-NIH | (Schwieters et al., 2003) | https://nmr.cit.nih.gov/xplor-nih/ |
| TALOS+ | (Shen et al., 2009) | https://spin.niddk.nih.gov/bax/nmrserver/talos/ |
| AMBER 11 | (Case et al., 2005) | https://ambermd.org/GetAmber.php |
| CLUSTERPOSE | (Diamond, 1995) | https://www.ccpn.ac.uk/v2-software/software/extras/contributions |
| PROCHECK-NMR | (Laskowski et al., 1993; Laskowski et al., 1996) | https://www.ebi.ac.uk/thornton-srv/software/PROCHECK/ |
| DeltaVision softWoRx | GE Healthcare Life Sciences | N/A |
| Other | ||
| Sephacryl S-300 column | GE Healthcare Life Sciences | Cat# GE17-0599-01 |
| Hi-Trap Q-FF column | GE Healthcare Life Sciences | Cat# 17515601 |
| Hi-Trap Q-HP column | GE Healthcare Life Sciences | Cat# 17115401 |
| Superdex S75 HR 10/30 column | GE Healthcare Life Sciences | Cat# 17-1047-01 |
| Hi-Trap SP-FF column | GE Healthcare Life Sciences | Cat# 17-5157-01 |
| Ni-NTA | Qiagen | Cat# 30210 |
| Complete Protease Inhibitor cocktail (EDTA free) | Roche/Merck | Cat# 11873580001 |
| Malvern Panalytical autoITC | Malvern | MRC-LMB |
| Jasco 815 Spectropolarimeter | JASCO | MRC-LMB |
| Bruker Avance-III spectrometers operating at 1H frequencies of 600, 700 or 800 MHz | Bruker | MRC-LMB |
| DeltaVision Elite microscope | GE Healthcare Life Sciences | National Centre for Biomolecular Research, Faculty of Science, Masaryk University |
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