FIG 2.

Order of infection does not impact the effect of symbiont colonization on pathogen persistence and intensity. (a) The proportion of individual bugs that tested positive for S. marcescens at a given time point. Pathogen-only bugs remained aposymbiotic, but bugs in the pathogen-first and symbiont-first treatment were coinfected with symbiont and pathogen. Each point represents 10 individuals tested at that time point. (b) S. marcescens titer recovered from infected squash bugs over time. Only nonzero values were included in this analysis. Points represent up to 10 individuals in each treatment, with later time points typically representing fewer individuals. In both panels, shading indicates 95% confidence intervals where possible to calculate.