FIG 3.

Time-kill assay of different A. baumannii strains, MK34 (A), RUH134 (B), Greek46 (C), and Greek47 (D), resuspended in high-tonicity buffer (20 mM HEPES-NaOH, 150 mM NaCl, pH 7.4; ∼2 × 10⁵ CFU/ml), resulting in a low intracellular osmotic pressure, upon exposure to 1 μM LysMK34 (red bars) or 1 μM eLysMK34 (blue bars). Green bars show the untreated cells. In the cases of the light-red and light-blue bars, 0.5 mM EDTA was included along with LysMK34 and eLysMK34, respectively. The numbers of surviving bacteria are expressed as log numbers of CFU per milliliter after 30, 60, and 120 min of exposure. Each value represents the mean ± standard deviation from three independent replicates. Asterisks represent statistical differences from values for untreated cells (Student's t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001). The dashed line represents the limit of detection (10 CFU/ml).