FIG 5.

Antibacterial activity of LysMK34 (red bars) and eLysMK34 (blue bars) against A. baumannii strains MK34 (A), RUH134 (B), Greek46 (C), and Greek47 (D) resuspended in 100% complement-deactivated human serum. The bacterial cells were treated with equimolar concentrations for 2 h. The treated cells were then washed twice and diluted prior to being plated in either complement-deactivated human serum (left) or 20 mM HEPES-NaOH, pH 7.4 (right). Green bars show the untreated cells. Each value represents the mean ± standard deviation from three independent replicates. Asterisks represent statistical differences from values for buffer-treated cells (Student's t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001). The dashed line represents the limit of detection (10 CFU/ml).