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The CTD in GFP-CTDCHU_2708 is necessary for its secretion by the T9SS in C. hutchinsonii. The periplasmic (P), outer membrane (OM), and extracellular (Extra) proteins of the different C. hutchinsonii expression strains were identified by Western blotting with anti-GFP antibody. The wild type (WT) was used as the control. The amount of protein loaded in each lane came from cells of equal culture volumes.
