TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| E. coli strains | ||
| BL21 | Strain with vector pUT-miniTn5-gfp-tet used for GFP gene cloning | TaKaRa |
| DH5α | Strain used for plasmid replication | TaKaRa |
| C. hutchinsonii strains | ||
| ATCC 33406 | Wild type | ATCC |
| WTGFP strain | WT containing promoterCHU_2708-signal peptideCHU_2708-GFP | This study |
| WTGFP-CTD strain | WT containing promoterCHU_2708-signal peptideCHU_2708-GFP-CTDCHU_2708 | This study |
| CHU_2708His strain | WT with His-tagged CHU_2708 | This study |
| N273Q mutant | Site-directed mutation of N273 of WTGFP-CTD | This study |
| N276Q mutant | Site-directed mutation of N276 of WTGFP-CTD | This study |
| T278A mutant | Site-directed mutation of T278 of WTGFP-CTD | This study |
| N276A mutant | Site-directed mutation of N276 of WTGFP-CTD | This study |
| N296Q mutant | Site-directed mutation of N296 of WTGFP-CTD | This study |
| C3842GFP-CTD strain | C3842 containing promoterCHU_2708-signal peptideCHU_2708-GFP-CTDCHU_2708 | This study |
| Δ3842 strain | chu_3842 deleted | This study |
| C3842 strain | Complementation of Δ3842 | This study |
| Plasmids | ||
| pTSK | Gene deletion template plasmid with ermF flanked by two FRT sites; Apr Emr | 17 |
| pTSK3328 | Plasmid for deletion of chu_3328; Apr Emr | 20 |
| pCFXSK3328 | Plasmid for deletion of chu_3328; Apr Cfxr | 59 |
| pCHF | Plasmid for complementation of Δ 3842; Apr Cmr | 17 |
a
Apr, ampicillin resistance; Emr, erythromycin resistance; Cfxr, cefoxitin resistance; Cmr, chloramphenicol resistance.