FIG 3.

Three laccases (A) and transcription factor Skn7 (B) were upregulated in expression in separated coculture of C. cinerea and Gongronella sp. w5 but ROS scavenger N-acetyl-l-cysteine (NAC) inhibited increase of skn7 transcription (C). C. cinerea mycelia were collected every 12 h during separated coculturing with Gongronella sp. w5. Then they were ground, extracted for RNA, and analyzed for transcriptional levels by qRT-PCR. The transcription levels of genes of monocultures without NAC (A and B) or at 0 h of separated coculture in SAHX with sucrose (C) were set as baselines. The data in panel C were detected at 24 h of separated coculture and analyzed using Student's t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). Data in panels A to C show mean ± SD, n = 3.