FIG 4.

ROS was involved in the synthesis of laccase during C. cinerea-Gongronella sp. w5 interspecific interaction in separated coculture. (A, B) ROS increased in C. cinerea separated cocultured with Gongronella sp. w5 in SAHX with sucrose (A) and added ROS scavenger NAC decreased laccase activity (A) and transcripts (B) of C. cinerea. Control represented separated cocultures without NAC. (C) NAC decreased laccase activity in monoculture of C. cinerea in SAHX medium using fructose as carbon source with 0.1 mM HBA added. Solid lines and dotted lines represent ROS values and laccase activities, respectively. The data were analyzed using Student's t test (*, P < 0.05; **, P < 0.01). Data show mean ± SD, n = 3. RFU, relative fluorescence units.